产品基本信息

• 产品货号 BD-PP0653
• 别名 ADRBK1; BARK; BARK1; GRK2; Beta-adrenergic receptor kinase 1; Beta-ARK-1; G-protein coupled receptor kinase 2
• 产品名称 GRK 2 (phospho Ser29) Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 ADRBK1
• 蛋白名称 Beta-adrenergic receptor kinase 1
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 156
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=156
• Human Swissprot No. P25098
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P25098/entry
• Mouse Swissprot No. Q99MK8
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q99MK8
• Rat Gene ID 25238
• Rat Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25238
• Rat Swissprot No. P26817
• Rat Swissprot Link http://www.uniprot.org/uniprot/P26817
• 免疫原 The antiserum was produced against synthesized peptide derived from human GRK2 around the phosphorylation site of Ser29. AA range:14-63
• 特异性 Phospho-GRK 2 (S29) Polyclonal Antibody detects endogenous levels of GRK 2 protein only when phosphorylated at S29.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:10000, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 80kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq, Jul 2008],
• 组织表达 Expressed in peripheral blood leukocytes.
• 细胞定位 Cytoplasm . Cell membrane . Cell junction, synapse, postsynapse . Cell junction, synapse, presynapse .
• 信号通路 Chemokine;Endocytosis;
• 功能 catalytic activity:ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate.,catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them.,online information:Beta adrenergic receptor kinase entry,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 RGS domain.,subunit:Interacts with GIT1 (By similarity). Interacts with, and phosphorylates chemokine-stimulated CCR5.,tissue specificity:Expressed in peripheral blood leukocytes.,
• 期货 现货
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using GRK2 （Phospho-Ser29） Antibody
	Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GRK2 （Phospho-Ser29） Antibody. The picture on the right is blocked with the phospho peptide.
	Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30‘, using GRK2 （Phospho-Ser29） Antibody. The lane on the right is blocked with the phospho peptide.

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