产品基本信息

• 产品货号 BD-PP0350
• 别名 ICAM3; Intercellular adhesion molecule 3; ICAM-3; CDw50; ICAM-R; CD antigen CD50
• 产品名称 CD50 (phospho Ser518) Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 ICAM3
• 蛋白名称 Intercellular adhesion molecule 3
• Clonality Polyclonal
• 推荐应用 WB,ELISA
• 反应种属 Human,Rat,Mouse
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 3385
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3385
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P32942/entry
• 免疫原 The antiserum was produced against synthesized peptide derived from human CD50/ICAM-3 around the phosphorylation site of Ser518. AA range:484-533
• 特异性 Phospho-CD50 (S518) Polyclonal Antibody detects endogenous levels of CD50 protein only when phosphorylated at S518.
• 稀释度 WB 1:500-1:2000, ELISA 1:40000.Not yet tested in other applications.
• 预测分子量 42-60kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2016],
• 组织表达 Leukocytes.
• 细胞定位 Membrane; Single-pass type I membrane protein.
• 信号通路 Cell adhesion molecules (CAMs);
• 功能 function:ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2.,online information:ICAM-3,PTM:N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains.,PTM:Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues.,similarity:Belongs to the immunoglobulin superfamily. ICAM family.,similarity:Contains 5 Ig-like C2-type (immunoglobulin-like) domains.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Western blot analysis of CD50/ICAM-3 （Phospho-Ser518） Antibody. The lane on the right is blocked with the CD50/ICAM-3 （Phospho-Ser518） peptide.
	Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using CD50/ICAM-3 （Phospho-Ser518） Antibody

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