产品基本信息

• 产品货号 BD-PP0326
• 别名 IRF3; Interferon regulatory factor 3; IRF-3
• 产品名称 IRF-3 (phospho Ser396) Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 IRF3
• 蛋白名称 Interferon regulatory factor 3
• Clonality Polyclonal
• 推荐应用 WB,IHC-P
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 3661
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3661
• Human Swissprot Link http://www.uniprot.org/uniprotkb/Q14653/entry
• Mouse Gene ID 54131
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=54131
• Mouse Swissprot No. P70671
• Mouse Swissprot Link http://www.uniprot.org/uniprot/P70671
• 免疫原 The antiserum was produced against synthesized peptide derived from human IRF-3 around the phosphorylation site of Ser396. AA range:362-411
• 特异性 Phospho-IRF-3 (S396) Polyclonal Antibody detects endogenous levels of IRF-3 protein only when phosphorylated at S396.
• 稀释度 WB 1:500-2000, IHC-P 1:50-300
• 预测分子量 48-55kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011],
• 组织表达 Expressed constitutively in a variety of tissues.
• 细胞定位 Cytoplasm . Nucleus . Mitochondrion . Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect (PubMed:10805757). When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm (PubMed:10805757). Recruited to mitochondria via TOMM70:HSP90AA1 upon Sendai virus infection (PubMed:25609812). .
• 信号通路 Toll_Like;RIG-I-like receptor;Cytosolic DNA-sensing pathway;
• 功能 function:Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains.,PTM:Constitutively phosphorylated on many serines residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Ser-385 and Ser-386 may be specifically phosphorylated in response to induction. An alternate model propose that the five serine/threonine residues between 396 and 405 are phosphorylated in response to a viral infection. Phosphorylation, and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3.,similarity:Belongs to the IRF family.,similarity:Contains 1 tryptophan pentad repeat DNA-binding domain.,subcellular location:Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.,subunit:Homodimer; phosphorylation-induced. Interacts with CREBBP. May interact with MAVS. Interacts with IKBKE and TBK1. Interacts with TICAM1 and TICAM2. Interacts with rotavirus A NSP1 (via C-terminus); this interaction leads to the proteasome-dependent degradation of IRF3.,tissue specificity:Expressed constitutively in a variety of tissues.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using IRF-3 （Phospho-Ser396） Antibody
	Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30‘, using IRF-3 （Phospho-Ser396） Antibody. The lane on the right is blocked with the phospho peptide.
	Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

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