产品基本信息

• 产品货号 BD-PP0280
• 别名 EIF4B; Eukaryotic translation initiation factor 4B; eIF-4B
• 产品名称 eIF4B (phospho Ser422) Rabbit Polyclonal Antibody
• 类别 抗体产品
• 基因名称 EIF4B
• 蛋白名称 Eukaryotic translation initiation factor 4B
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 3716
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3716
• Human Swissprot No. P23588
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P23588/entry
• Mouse Gene ID 75705
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=75705
• Mouse Swissprot No. Q8BGD9
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q8BGD9
• 免疫原 The antiserum was produced against synthesized peptide derived from human eIF4B around the phosphorylation site of Ser422. AA range:388-437
• 特异性 Phospho-eIF4B (S422) Polyclonal Antibody detects endogenous levels of eIF4B protein only when phosphorylated at S422.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, IF-P/IF-F/IF-ICC 1:200-1:1000, ELISA 1:40000.Not yet tested in other applications.
• 预测分子量 80kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 function:Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Self-associates and interacts with EIF3 p170 subunit.,
• 组织表达 Adipose tissue,Brain,Cervix carcinoma,Epithelium,Liver,Lung
• 细胞定位 cytosol,polysome,eukaryotic translation initiation factor 4F complex,dendrite,neuronal cell body,
• 信号通路 mTOR;
• 功能 function:Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Self-associates and interacts with EIF3 p170 subunit.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using eIF4B （Phospho-Ser422） Antibody
	Immunofluorescence analysis of NIH/3T3 cells, using eIF4B （Phospho-Ser422） Antibody. The picture on the right is blocked with the phospho peptide.
	Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using eIF4B （Phospho-Ser422） Antibody. The picture on the right is blocked with the phospho peptide.
	Western blot analysis of lysates from NIH/3T3 cells, using eIF4B （Phospho-Ser422） Antibody. The lane on the right is blocked with the phospho peptide.
	Western Blot analysis of 293 cells using Phospho-eIF4B （S422） Polyclonal Antibody diluted at 1：2000

产品详情