产品基本信息

• 产品货号 BD-PP0256
• 别名 STAT6; Signal transducer and activator of transcription 6; IL-4 Stat
• 产品名称 Stat6 (phospho Tyr641) Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 STAT6
• 蛋白名称 Signal transducer and activator of transcription 6
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 6778
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6778
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P42226/entry
• Mouse Gene ID 20852
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=20852
• Mouse Swissprot No. P52633
• Mouse Swissprot Link http://www.uniprot.org/uniprot/P52633
• 免疫原 The antiserum was produced against synthesized peptide derived from human STAT6 around the phosphorylation site of Tyr641. AA range:608-657
• 特异性 Phospho-Stat6 (Y641) Polyclonal Antibody detects endogenous levels of Stat6 protein only when phosphorylated at Y641.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:10000, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 94kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010],
• 组织表达 Uterus,
• 细胞定位 Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
• 信号通路 Jak_STAT;
• 功能 function:Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.,PTM:Tyrosine phosphorylated following stimulation by IL-4 and IL-3.,similarity:Belongs to the transcription factor STAT family.,similarity:Contains 1 SH2 domain.,subcellular location:Translocated into the nucleus in response to phosphorylation.,subunit:Forms a homodimer or a heterodimer with a related family member (By similarity). Interacts with NCOA1 via its C-terminal LXXLL motif.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using STAT6 （Phospho-Tyr641） Antibody
	Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT6 （Phospho-Tyr641） Antibody. The picture on the right is blocked with the phospho peptide.
	Western blot analysis of lysates from HeLa cells treated with IL-4, using STAT6 （Phospho-Tyr641） Antibody. The lane on the right is blocked with the phospho peptide.
	Western Blot analysis of various cells using Phospho-Stat6 （Y641） Polyclonal Antibody
	Western blot analysis of Stat6 (phospho Tyr641) Polyclonal Antibody, using Hela,Jurkat cell treated or untreated with IFN 2500U/ml 30', 4° over night, secondary antibody was diluted at 1:10000, 37° 1hour.

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