产品基本信息

• 产品货号 BD-PP0141
• 别名 CHUK; IKKA; TCF16; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B
• 产品名称 IKKα/β (phospho Ser176/177) Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 CHUK/IKBKB
• 蛋白名称 Inhibitor of nuclear factor kappa-B kinase subunit alpha
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 1147/3551
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1147
• Human Swissprot Link http://www.uniprot.org/uniprotkb/O15111/entry
• Mouse Gene ID 16150
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=16150
• Rat Gene ID 84351
• Rat Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=84351
• Rat Swissprot No. Q9QY78
• Rat Swissprot Link http://www.uniprot.org/uniprot/Q9QY78
• 免疫原 The antiserum was produced against synthesized peptide derived from human IKK-alpha around the phosphorylation site of Ser177. AA range:151-200
• 特异性 Phospho-IKKα/β (S176/177) Polyclonal Antibody detects endogenous levels of IKKα/β protein only when phosphorylated at S176/177.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:10000, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 80kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008],
• 组织表达 Widely expressed.
• 细胞定位 Cytoplasm . Nucleus . Shuttles between the cytoplasm and the nucleus.
• 信号通路 T_Cell_Receptor; Insulin Receptor; B_Cell_Antigen; Stem cell pathway; Toll_Like; MAPK_ERK_Growth;MAPK_G_Protein; PI3K/Akt; NF_kappaB; Protein_Acetylation
• 功能 catalytic activity:ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].,enzyme regulation:Activated when phosphorylated and inactivated when dephosphorylated.,function:Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.,PTM:Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:Shuttles between the cytoplasm and the nucleus.,subunit:Component of the I-kappa-B-kinase (IKK) core complex consisting of CHUK, IKBKB and IKBKG; probably four alpha/CHUK-beta/IKBKB dimers are associated with four gamma/IKBKG subunits. The IKK core complex seems to associate with regulatory or adapter proteins to form a IKK-signalosome holo-complex. Part of a complex composed of NCOA2, NCOA3, CHUK/IKKA, IKBKB, IKBKG and CREBBP. Part of a 70-90 kDa complex at least consisting of CHUK/IKKA, IKBKB, NFKBIA, RELA, IKBKAP and MAP3K14. Directly interacts with IKK-gamma/NEMO and TRPC4AP (By similarity). May interact with TRAF2. Interacts with NALP2. May interact with MAVS/IPS1.,tissue specificity:Widely expressed.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Immunohistochemistry analysis of paraffin-embedded human heart, using IKK-alpha （Phospho-Ser176） /IKK-beta （Phospho-Ser177） Antibody. The picture on the right is blocked with the phospho peptide.
	Western blot analysis of lysates from NIH/3T3 cells treated with TNF 20ng/ml 30‘, using IKK-alpha （Phospho-Ser176） /IKK-beta （Phospho-Ser177） Antibody. The lane on the right is blocked with the phospho peptide.
	Western Blot analysis of NIH-3T3 cells using Phospho-IKKα/β （S176/177） Polyclonal Antibody diluted at 1：1000
	The picture was kindly provided by our customer,antibody was diluted at 1:500

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