产品基本信息

• 产品货号 BD-PP0117
• 别名 G3BP1; G3BP; Ras GTPase-activating protein-binding protein 1; G3BP-1; ATP-dependent DNA helicase VIII; hDH VIII; GAP SH3 domain-binding protein 1
• 产品名称 G3BP1 (phospho Ser232) Rabbit Polyclonal Antibody
• 类别 抗体产品
• 基因名称 G3BP1
• 蛋白名称 Ras GTPase-activating protein-binding protein 1
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 10146
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=10146
• Human Swissprot No. Q13283
• Human Swissprot Link http://www.uniprot.org/uniprotkb/Q13283/entry
• Mouse Gene ID 27041
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=27041
• Mouse Swissprot No. P97855
• Mouse Swissprot Link http://www.uniprot.org/uniprot/P97855
• 免疫原 The antiserum was produced against synthesized peptide derived from human G3BP-1 around the phosphorylation site of Ser232. AA range:216-248
• 特异性 Phospho-G3BP1 (S232) Polyclonal Antibody detects endogenous levels of G3BP1 protein only when phosphorylated at S232.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:5000, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 60kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008],
• 组织表达 Ubiquitous.
• 细胞定位 Cytoplasm, cytosol . Perikaryon . Cytoplasm, Stress granule . Nucleus . Cytoplasmic in proliferating cells (PubMed:11604510). Cytosolic and partially nuclear in resting cells (PubMed:11604510). Recruited to stress granules in response to arsenite treatment (PubMed:12642610, PubMed:20180778). The unphosphorylated form is recruited to stress granules (PubMed:12642610). HRAS signaling contributes to this process by regulating G3BP dephosphorylation (PubMed:12642610). .
• 功能 cofactor:Magnesium. Required for helicase activity.,domain:The NTF2 domain mediates multimerization.,function:May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.,PTM:Arg-435 is dimethylated, probably to asymmetric dimethylarginine.,PTM:Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization.,similarity:Contains 1 NTF2 domain.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subcellular location:Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells (By similarity). Cytosolic and partially nuclear in resting cells. Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS.,subunit:Binds to the SH3 domain of Ras GTPase-activating protein (RASA1) in proliferating cells. No interaction in quiescent cells Component of a TAU mRNP complex, at least composed of IGF2BP1, ELAVL4 and G3BP (By similarity). Interacts with USP10, and may regulate it. Forms homodimers and oligomers.,tissue specificity:Ubiquitous.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Immunohistochemistry analysis of paraffin-embedded human breast cancer, using G3BP-1 （Phospho-Ser232） Antibody. The picture on the right is blocked with the G3BP-1 （Phospho-Ser232） peptide.
	Western blot analysis of extracts from HeLa cells, using G3BP-1 （Phospho-Ser232） Antibody. The lane on the right is treated with the synthesized peptide.
	Western Blot analysis of various cells using Phospho-G3BP1 （S232） Polyclonal Antibody

产品详情