产品基本信息

• 产品货号 BD-PN1888
• 产品名称 TRIO Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 TRIO
• 蛋白名称 Triple functional domain protein (EC 2.7.11.1) (PTPRF-interacting protein)
• Clonality Polyclonal
• 推荐应用 IHC-P,IF-P,IF-F,IF-ICC
• 反应种属 Human,Mouse
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
• Human Gene ID 7204
• Human Swissprot Link https://www.uniprot.org/uniprotkb/O75962/entry
• Mouse Swissprot No. Q0KL02
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q0KL02
• 免疫原 Synthesized peptide derived from part region of human protein
• 特异性 TRIO Polyclonal Antibody detects endogenous levels of protein.
• 稀释度 IHC-P 1:50-300, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 340kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene encodes a large protein that functions as a GDP to GTP exchange factor. This protein promotes the reorganization of the actin cytoskeleton, thereby playing a role in cell migration and growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015],
• 组织表达 Widely expressed, with highest levels in heart, skeletal muscle, and brain.
• 细胞定位 Cytoplasm . Cell projection .
• 功能 catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The N-terminal DBL/GEF domain specifically catalyzes nucleotide exchange for RAC1, leading to the activation of Jun kinase and the production of membrane ruffles. The second DBL/GEF domain is an exchange factor for rhoa and induces the formation of stress fibers.,function:Promotes the exchange of GDP by GTP. Together with leukocyte antigen-related (LAR) protein, it could play a role in coordinating cell-matrix and cytoskeletal rearrangements necessary for cell migration and cell growth.,PTM:Phosphorylated on serine residue(s).,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.,similarity:Contains 1 CRAL-TRIO domain.,similarity:Contains 1 Ig-like C2-type (immunoglobulin-like) domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 DH (DBL-homology) domains.,similarity:Contains 2 PH domains.,similarity:Contains 2 SH3 domains.,similarity:Contains 4 spectrin repeats.,subunit:Interacts to form a complex with leukocyte antigen related protein.,tissue specificity:Highly expressed in heart, skeletal muscle, brain, pancreas, placenta, liver, kidney and lung.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200（4° overnight）. 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

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