产品基本信息

• 产品货号 BD-PN0881
• 产品名称 MUC5B Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 MUC5B MUC5
• 蛋白名称 Mucin-5B (MUC-5B) (Cervical mucin) (High molecular weight salivary mucin MG1) (Mucin-5 subtype B, tracheobronchial) (Sublingual gland mucin)
• Clonality Polyclonal
• 推荐应用 IHC-P,IF-P,IF-F,IF-ICC
• 反应种属 Human,Rat,Mouse
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
• Human Gene ID 727897
• Human Swissprot No. Q9HC84
• Human Swissprot Link https://www.uniprot.org/uniprotkb/Q9HC84/entry
• 免疫原 Synthesized peptide derived from human protein . at AA range: 1810-1890
• 特异性 MUC5B Polyclonal Antibody detects endogenous levels of protein.
• 稀释度 IHC-P 1:50-300, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 633kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene encodes a member of the mucin family of proteins, which are highly glycosylated macromolecular components of mucus secretions. This family member is the major gel-forming mucin in mucus. It is a major contributor to the lubricating and viscoelastic properties of whole saliva, normal lung mucus and cervical mucus. This gene has been found to be up-regulated in some human diseases, including sinus mucosa of chronic rhinosinusitis (CRS), CRS with nasal polyposis, chronic obstructive pulmonary disease (COPD) and H. pylori-associated gastric disease, and it may be involved in the pathogenesis of these diseases. [provided by RefSeq, Jul 2010],
• 组织表达 Expressed on surface airway epithelia. Expressed mainly in mucous cells of submucosal glands of airway tissues. Highly expressed in the sublingual gland. Also found in submaxillary glands, endocervix, gall bladder, and pancreas.
• 细胞定位 Secreted.
• 功能 domain:The cysteine residues in the Cys-rich subdomain repeats are not involved in disulfide bonding.,function:Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus.,induction:Regulated by all-trans-retinoic acid in a cell-type specific manner.,PTM:Highly glycosylated. C-, N- and O-gylcosylated. C-mannosylated in the Cys-rich subdomains probably on the first Trp residue of the WXXW motif. Highly O-glycosylated in the Ser/Thr-rich tandem repeat (TR) region. The repeat region is about 59% O-glycosylated with a high abundance of NeuAc(2)Hex(1)HexNac1-ol.,similarity:Contains 1 CTCK (C-terminal cystine knot-like) domain.,similarity:Contains 1 TIL (trypsin inhibitory-like) domain.,similarity:Contains 3 VWFC domains.,similarity:Contains 4 VWFD domains.,tissue specificity:Expressed on surface airway epithelia. Expressed mainly in mucous cells of submucosal glands of airway tissues. Highly expressed in the sublingual gland. Also found in submaxillary glands, endocervix, gall bladder, and pancreas.,
• 期货 现货
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200（4° overnight）. 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

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