产品基本信息

• 产品货号 BD-PC0001
• 别名 ACR; ACRS; Acrosin
• 产品名称 Acrosin Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 ACR
• 蛋白名称 Acrosin
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Rat,Mouse
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 49
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=49
• Human Swissprot No. P10323
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P10323/entry
• Mouse Swissprot No. P23578
• Mouse Swissprot Link http://www.uniprot.org/uniprot/P23578
• 免疫原 Synthesized peptide derived from Acrosin . at AA range: 40-120
• 特异性 Acrosin Polyclonal Antibody detects endogenous levels of Acrosin protein.
• 稀释度 IHC-P 100-300.WB 1:500-1:2000, ELISA 1:5000, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 45kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids. [provided by RefSeq, Jul 2008],
• 组织表达 Leukocyte,Testis,
• 细胞定位 acrosomal vesicle,extracellular region,nucleus,Golgi-associated vesicle,acrosomal matrix,protein complex,
• 功能 catalytic activity:Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.,function:Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.,similarity:Belongs to the peptidase S1 family.,similarity:Contains 1 peptidase S1 domain.,subunit:Heavy chain (catalytic) and a light chain linked by two disulfide bonds.,
• 期货 现货
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Western Blot analysis of various cells using Acrosin Polyclonal Antibody
	Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

产品详情