纯化The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
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Western blot analysis of 1 HEK293 2 SW480 3 HEPG2 4 MCF-7 5 mouse brain 6 Rat brain 7 Hela 8 A549 lysates, primary antibody was diluted at 1:5000, 4° over night, secondary antibody(cat: RS23910 was diluted at 1:10000, 37° 1hour.