Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Transferrin Monoclonal Antibody(7F4) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. | |
Immunofluorescence analysis of Human-lung-cancer tissue. 1,Transferrin Monoclonal Antibody(7F4)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B | |
Western blot analysis of Human serum, mAb diluted at 1:2000. |