All lanes : Anti-(DANRE) atf4 Antibody(C-term) at 1:1000 dilution Lane 1: Zebrafish lysate Lane 2: Zebrafish muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |