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45 μg rat heart tissue lysate was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. BDAM1512 at 1 µg/mL was used as the primary antibody. TNNI3 band was visualized using ECL Western Blotting Substrate. Result: BDAM1512 can detect TNNI3 by Western blotting. |