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45 μg of HeLa lysate and 45 μg of NIH/3T3 lysate were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. BDAM1502 at 1.00 µg/mL was used as the primary antibody. ENO1 band was visualized using ECL Western Blotting Substrate. Lane 1: 45 μg of HeLa lysate Lane 2: 45 μg of NIH/3T3 lysate Result: BDAM1502 can detect ENO1 by Western blotting. |