产品基本信息

• 产品货号 BD-PT1213
• 别名 CYP2C8; Cytochrome P450 2C8; CYPIIC8; Cytochrome P450 IIC2; Cytochrome P450 MP-12; Cytochrome P450 MP-20; Cytochrome P450 form 1; S-mephenytoin 4-hydroxylase; CYP2C9; CYP2C10; Cytochrome P450 2C9; (R)-limonene 6-monooxygenase; (S)-limonene
• 产品名称 CYP2C8/9/18/19 Rabbit Polyclonal Antibody
• 类别 抗体产品
• 基因名称 CYP2C8/9/18/19
• 蛋白名称 Cytochrome P450 2C8/9/18/19
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Rat,Mouse
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 1562/1558/1559/1557
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1562
• Human Swissprot No. P10632/P11712/P33260/P33261
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P10632/entry
• 免疫原 The antiserum was produced against synthesized peptide derived from human Cytochrome P450 2C8/9/18/19. AA range:111-160
• 特异性 CYP2C8/9/18/19 Polyclonal Antibody detects endogenous levels of CYP2C8/9/18/19 protein.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, IF-P/IF-F/IF-ICC 1:200-1:1000, ELISA 1:20000.Not yet tested in other applications.
• 预测分子量 60kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize many xenobiotics, including the anticonvulsive drug mephenytoin, benzo(a)pyrene, 7-ethyoxycoumarin, and the anti-cancer drug taxol. This gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Nov 2010],
• 组织表达 Blood,Kidney,Liver,
• 细胞定位 Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
• 信号通路 Arachidonic acid metabolism;Linoleic acid metabolism;Retinol metabolism;Metabolism of xenobiotics by cytochrome P450;Drug metabolism;
• 功能 catalytic activity:RH + reduced flavoprotein + O(2) = ROH + oxidized flavoprotein + H(2)O.,caution:Alternative splicing has been shown to occur but the shorter forms are believed to be non-functional.,cofactor:Heme group.,function:Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. In the epoxidation of arachidonic acid it generates only 14,15- and 11,12-cis-epoxyeicosatrienoic acids. It is the principal enzyme responsible for the metabolism the anti-cancer drug paclitaxel (taxol).,induction:By phenobarbital.,online information:CYP2C8 alleles,similarity:Belongs to the cytochrome P450 family.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Western blot analysis of lysates from LOVO cells, using Cytochrome P450 2C8/9/18/19 Antibody. The lane on the right is blocked with the synthesized peptide.
	Western Blot analysis of LOVO cells using CYP2C8/9/18/19 Polyclonal Antibody
	Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

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