产品基本信息

• 产品货号 BD-PP0678
• 别名 FLT1; FLT; FRT; VEGFR1; Vascular endothelial growth factor receptor 1; VEGFR-1; Fms-like tyrosine kinase 1; FLT-1; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; FLT; Vascular permeability factor receptor
• 产品名称 Flt-1 (phospho Tyr1333) Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 FLT1
• 蛋白名称 Vascular endothelial growth factor receptor 1
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 2321
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2321
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P17948/entry
• Mouse Gene ID 14254
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=14254
• Mouse Swissprot No. P35969
• Mouse Swissprot Link http://www.uniprot.org/uniprot/P35969
• Rat Gene ID 54251
• Rat Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=54251
• Rat Swissprot No. P53767
• Rat Swissprot Link http://www.uniprot.org/uniprot/P53767
• 免疫原 The antiserum was produced against synthesized peptide derived from human VEGFR1 around the phosphorylation site of Tyr1333. AA range:1289-1338
• 特异性 Phospho-Flt-1 (Y1333) Polyclonal Antibody detects endogenous levels of Flt-1 protein only when phosphorylated at Y1333.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-300, ELISA 1:20000, IF-P/IF-F/IF-ICC 1:100-300, Not yet tested in other applications.
• 预测分子量 151kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene encodes a member of the vascular endothelial growth factor receptor (VEGFR) family. VEGFR family members are receptor tyrosine kinases (RTKs) which contain an extracellular ligand-binding region with seven immunoglobulin (Ig)-like domains, a transmembrane segment, and a tyrosine kinase (TK) domain within the cytoplasmic domain. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.[provided by RefSeq, May 2009],
• 组织表达 Detected in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform 2 is strongly expressed in placenta. Isoform 3 is expressed in corneal epithelial cells (at protein level). Isoform 3 is expressed in vascular smooth muscle cells (VSMC).
• 细胞定位 [Isoform 1]: Cell membrane; Single-pass type I membrane protein. Endosome. Autophosphorylation promotes ubiquitination and endocytosis.; [Isoform 2]: Secreted .; [Isoform 3]: Secreted.; [Isoform 4]: Secreted.; [Isoform 5]: Cytoplasm .; [Isoform 6]: Cytoplasm .; [Isoform 7]: Cytoplasm .
• 信号通路 Cytokine-cytokine receptor interaction;Endocytosis;Focal adhesion;
• 功能 alternative products:Additional isoforms seem to exist,catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 7 Ig-like C2-type (immunoglobulin-like) domains.,subunit:Interacts in vitro with various phosphotyrosine-binding proteins, including PLC-gammas, PTPN11, GRB2, CRK and NCK1.,tissue specificity:Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. It is not expressed in tumor cell lines. Isoform sFlt1 is strongly expressed in placenta.,
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using VEGFR1 （Phospho-Tyr1333） Antibody
	Immunohistochemistry analysis of paraffin-embedded human brain, using VEGFR1 （Phospho-Tyr1333） Antibody. The picture on the right is blocked with the phospho peptide.
	Western blot analysis of lysates from K562 cells treated with etoposide 25uM 24h, using VEGFR1 （Phospho-Tyr1333） Antibody. The lane on the right is blocked with the phospho peptide.
	Western Blot analysis of various cells using Phospho-Flt-1 （Y1333） Polyclonal Antibody
	Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.

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