产品基本信息

• 产品货号 BD-PP0145
• 别名 IRS1; Insulin receptor substrate 1; IRS-1
• 产品名称 IRS-1 (phospho Ser1101) Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 IRS1
• 蛋白名称 Insulin receptor substrate 1
• Clonality Polyclonal
• 推荐应用 WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
• 反应种属 Human,Mouse,Rat,Monkey
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 3667
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3667
• Human Swissprot No. P35568
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P35568/entry
• Mouse Gene ID 16367
• Mouse Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=16367
• Mouse Swissprot No. P35569
• Mouse Swissprot Link http://www.uniprot.org/uniprot/P35569
• Rat Gene ID 25467
• Rat Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25467
• Rat Swissprot No. P35570
• Rat Swissprot Link http://www.uniprot.org/uniprot/P35570
• 免疫原 The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Ser1101. AA range:1067-1116
• 特异性 Phospho-IRS-1 (S1101) Polyclonal Antibody detects endogenous levels of IRS-1 protein only when phosphorylated at S1101.
• 稀释度 WB 1:500-1:2000, IHC-P 1:100-1:300, ELISA 1:20000, IF-P/IF-F/IF-ICC 1:50-200
• 预测分子量 170kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009],
• 组织表达 Epithelium,Eye,Skeletal muscle,
• 细胞定位 nucleus,cytoplasm,cytosol,plasma membrane,insulin receptor complex,caveola,intracellular membrane-bounded organelle,
• 信号通路 Neurotrophin;Insulin_Receptor;Adipocytokine;Type II diabetes mellitus;Aldosterone-regulated sodium reabsorption;
• 功能 disease:Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].,function:May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.,polymorphism:The Arg-971 polymorphism impairs the ability of insulin to stimulate glucose transport, glucose transporter translocation, and glycogen synthesis by affecting the PI3K/AKT1/GSK3 signaling pathway. The polymorphism at Arg-971 may contribute to the in vivo insulin resistance observed in carriers of this variant. Arg-971 could contribute to the risk for atherosclerotic cardiovascular diseases associated with non-insulin-dependent diabetes mellitus (NIDDM) by producing a cluster of insulin resistance-related metabolic abnormalities. In insulin-stimulated human endothelial cells from carriers of the Arg-971 polymorphism, genetic impairment of the IRS1/PI3K/PDPK1/AKT1 insulin signaling cascade results in impaired insulin-stimulated nitric oxide (NO) release and suggested that this may be a mechanism through which the Arg-971 polymorphism contributes to the genetic predisposition to develop endothelial dysfunction and cardiovascular disease. The Arg-971 polymorphism not only reduces phosphorylation of the substrate but allows IRS1 to act as an inhibitor of PI3K, producing global insulin resistance.,PTM:Phosphorylation of Tyr-896 is required for GRB2-binding.,PTM:Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.,similarity:Contains 1 IRS-type PTB domain.,similarity:Contains 1 PH domain.,subunit:Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR via the PTB domain. Binds to phosphatidylinositol 3-kinase p85 subunit via the phosphorylated YXXM motifs. Binds ROCK1. Binds to UBTF and PIK3CA in nuclear extracts (By similarity). Interacts with SOCS7.,
• 期货 现货
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Enzyme-Linked Immunosorbent Assay （Phospho-ELISA） for Immunogen Phosphopeptide （Phospho-left） and Non-Phosphopeptide （Phospho-right）, using IRS-1 （Phospho-Ser1101） Antibody
	Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using IRS-1 （Phospho-Ser1101） Antibody. The picture on the right is blocked with the phospho peptide.
	Western blot analysis of lysates from COS7 cells treated with Calyculin A 50ng/ml 30‘, using IRS-1 （Phospho-Ser1101） Antibody. The lane on the right is blocked with the phospho peptide.
	Western Blot analysis of various cells using Phospho-IRS-1 （S1101） Polyclonal Antibody diluted at 1：500

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