产品基本信息

• 产品货号 BD-PC0085
• 别名 F12; Coagulation factor XII; Hageman factor; HAF
• 产品名称 Factor XII HC (Cleaved R372) Rabbit Polyclonal Antibody
• 类别 抗原抗体
• 基因名称 F12
• 蛋白名称 Coagulation factor XII
• Clonality Polyclonal
• 推荐应用 WB,ELISA
• 反应种属 Human,Rat,Mouse
• 浓度 1 mg/ml
• 存储缓冲液 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
• Human Gene ID 2161
• Human Gene Link http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2161
• Human Swissprot No. P00748
• Human Swissprot Link http://www.uniprot.org/uniprotkb/P00748/entry
• Mouse Swissprot No. Q80YC5
• Mouse Swissprot Link http://www.uniprot.org/uniprot/Q80YC5
• 免疫原 The antiserum was produced against synthesized peptide derived from human FA12. AA range:323-372
• 特异性 Cleaved-Factor XII HC (R372) Polyclonal Antibody detects endogenous levels of fragment of activated Factor XII HC protein resulting from cleavage adjacent to R372.
• 稀释度 WB 1:500-1:2000, ELISA 1:20000.Not yet tested in other applications.
• 预测分子量 41kDa
• 运输及保存条件 -20°C/1 year
• 宿主 Rabbit
• 同种型 Rabbit,IgG
• 背景介绍 This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activat
• 组织表达 Blood,Lung,Plasma,
• 细胞定位 Secreted.
• 信号通路 Complement and coagulation cascades;
• 功能 catalytic activity:Selective cleavage of Arg-|-Ile bonds in factor VII to form factor VIIa and factor XI to form factor XIa.,disease:Defects in F12 are the cause of factor XII deficiency (FA12D) [MIM:234000]; also known as Hageman factor deficiency. This trait is an asymptomatic anomaly of in vitro blood coagulation. Its diagnosis is based on finding a low plasma activity of the factor in coagulating assays. It is usually only accidentally discovered through pre-operative blood tests. F12 deficiency is divided into two categories, a cross-reacting material (CRM)-negative group (negative F12 antigen detection) and a CRM-positive group (positive F12 antigen detection).,disease:Defects in F12 are the cause of hereditary angioedema type 3 (HAE3) [MIM:610618]; also known as estrogen-related HAE or hereditary angioneurotic edema with normal C1 inhibitor concentration and function. HAE is characterized by episodic local subcutaneous edema, and submucosal edema involving the upper respiratory and gastrointestinal tracts. HAE3 occurs exclusively in women and is precipitated or worsened by high estrogen levels (e.g., during pregnancy or treatment with oral contraceptives). It differs from HAE types 1 and 2 in that both concentration and function of C1 inhibitor are normal.,function:Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa.,online information:F12 mutation db,online information:Factor XII entry,PTM:O- and N-glycosylated. The O-linked polysaccharides were not identified, but are probably the mucin type linked to GalNAc.,similarity:Belongs to the peptidase S1 family.,similarity:Contains 1 fibronectin type-I domain.,similarity:Contains 1 fibronectin type-II domain.,similarity:Contains 1 kringle domain.,similarity:Contains 1 peptidase S1 domain.,similarity:Contains 2 EGF-like domains.,
• 期货 现货
• 纯化 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

图片

	Western blot analysis of lysates from 293 cells, treated with etoposide 25uM 1h, using FA12 （heavy chain,Cleaved-Arg372） Antibody. The lane on the right is blocked with the synthesized peptide.
	Western Blot analysis of various cells using Cleaved-Factor XII HC （R372） Polyclonal Antibody

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